Figure 1.

BTK expression in prostate cell lines and tumors. BTK-C message is more abundant than the BTK-A isoform in prostate cancer cells and tumors. cDNA prepared from RNA isolated from prostate cancer cell lines (A) and human prostate normal and tumor tissues. (B) was subjected to qPCR using primers specific for BTK-A and BTK-C isoforms. Expression of each isoform was normalized to an actin control in the respective qPCR reaction. The data represent relative mRNA levels of each BTK isoform as fold increase of the normal tissue sample with the lowest expression. Lymphoma samples are shown for comparison. BTK-C was the predominant isoform in only 3% of lymphomas but 40% of prostate tumors (6 out of 15 cases).