Table 1.
Anthropometric, clinical and biochemical data (mean ± SD) of all individuals (n=120) categorized by quartiles of LINE-1 methylation (%).
| Variables | Q1< 77.01% | Q2 77.02 to 83.01% | Q3 83.02 to 89.05% | Q4 >89.06% | P-for linear trend |
|---|---|---|---|---|---|
| Age (y) | 23.2 (3.2) | 24.5 (4.1) | 23.0 (3.8) | 22.8 (3.0) | 0.280 |
| BMI (kg/m2) | 21.9 (2.0) | 22.6 (3.3) | 22.0 (2.7) | 22.4 (3.1) | 0.732 |
| Waist perimeter (cm) | 77.7 (8.2) | 80.5 (8.9) | 78.0 (7.5) | 78.9 (9.4) | 0.532 |
| Hip perimeter (cm) | 98.0 (4.9) | 97.5 (6.5)a | 95.8 (5.6)a | 93.1 (7.5)a,b | 0.013 |
| Waist-to-hip ratio | 0.79 (0.06) | 0.82 (0.06) | 0.81 (0.06) | 0.84 (0.06)a | 0.018 |
| Total body fat - BIA (%) | 23.6 (6.0) | 22.6 (7.0)b | 20.5 (6.7)b | 18.2 (7.8)a,b | <0.001 |
| Body fat mass - BIA(kg) | 18.6 (5.8) | 16.9 (7.0) | 14.6 (4.3) | 14.0 (5.2) | 0.080 |
| Truncal fat (%) | 56.7 (5.7) | 59.6 (7.3) | 59.0 (7.2) | 60.9 (6.4) | 0.052 |
| Sum of 4 STs (mm) | 68.9 (17.7) | 67.6 (24.8) | 60.0 (20.4) | 56.1 (30.3)a,b | 0.050 |
| Body free fat mass - BIA(kg) | 43.8 (8.4) | 47.0 (11.7) | 48.3 (9.9) | 51.3 (10.1)a,b | 0.016 |
| Systolic blood pressure (mmHg) | 10.7 (0.8) | 11.0 (0.6) | 10.9 (1.0) | 11.4 (1.0) | 0.038 |
| Diastolic blood pressure (mmHg) | 7.3 (0.6) | 7.3 (0.5) | 7.3 (0.7) | 7.5 (0.8) | 0.521 |
| Glucose (mg/dL) | 91.0 (8.0) | 91.8 (7.0) | 90.7 (6.8) | 90.5 (6.9) | 0.362 |
| Insulin (µU/mL) | 15.8 (2.8) | 17.2 (4.7) | 16.2 (3.9) | 13.4 (2.4) | 0.423 |
| HOMA-IR | 3.63 (0.67) | 4.06 (1.09) | 3.87 (1.09) | 3.14 (0.48) | 0.215 |
| Total cholesterol (mg/dL) | 162.4 (29.6) | 162.7 (21.6) | 158.7 (35.8) | 151.1 (32.8) | 0.426 |
| HDL-c (mg/dL) | 48.8 (7.6) | 45.6 (11.2) | 43.7 (10.1) | 42.4 (10.3) | 0.087 |
| LDL-c (mg/dL) | 91.8 (23.5) | 91.7 (18.0) | 96.2 (30.1) | 91.6 (26.5) | 0.890 |
| VLDL-c (mg/dL) | 20.5 (7.8) | 20.7 (9.1) | 20.1 (7.3) | 17.0 (5.8) | 0.218 |
| Triacylglycerol (mg/dL) | 102.7 (39.4) | 108.6 (52.3) | 100.9 (36.5) | 85.4 (29.1) | 0.063 |
| Total cholesterol-to-HDL-c ratio | 3.31 (0.54) | 3.60 (0.80) | 3.74 (0.86) | 3.56 (0.69) | 0.971 |
| CRP-hs | 2.00 (2.21) | 1.81 (1.58) | 1.74 (2.64) | 0.99 (1.37) | 0.224 |
| IL-6 (pg/mL) | 15.8 (14.8) | 21.6 (22.5) | 19.8 (15.5) | 35.5 (21.0)a,b | 0.006 |
| TNF-α (pg/mL) | 4.13 (1.96) | 4.45 (1.58) | 4.21 (2.01) | 5.05 (2.75) | 0.331 |
| C3 complement | 110.3 (24.6) | 110.8 (25.3) | 114.3 (25.6) | 110.3 (21.0) | 0.904 |
| Ceruloplasmin (mg/dL) | 40.37 (8.40) | 36.87 (7.91) | 36.50 (7.82) | 32.51 (5.98)a,b | 0.002 |
| Adiponectin | 32.7 (10.3) | 32.4 (22.9) | 22.1 (9.0) | 23.1 (8.8) | 0.294 |
| GPx activity (nmol/[ml/min]) | 576 (310) | 540 (267) | 542 (279) | 643 (251) | 0.580 |
| Total antioxidant capacity (mM) | 1.50 (0.80) | 1.76 (0.94) | 1.46 (0.72) | 1.89 (1.07) | 0.196 |
| Ox-LDL (U/L) | 62.7 (28.0) | 67.7 (22.1) | 80.7 (31.8) | 76.2 (32.4) | 0.081 |
| Selenium (ng/g of nail) | 0.41 (0.07) | 0.40 (0.08) | 0.39 (0.09) | 0.35 (0.06) | 0.109 |
| Copper (ng/g of nail) | 6.97 (4.10) | 6.42 (2.90) | 7.39 (5.70) | 7.04 (4.50) | 0.894 |
| Zinc (ng/g of nail) | 124.8 (25.0) | 114.8 (16.8) | 146.0 (99.0) | 128.4 (71.7) | 0.376 |
BIA: Electrical bioimpedance; HOMA-IR: Homeostatic model assessment; CRP-hs: High-sensitivity C-reactive protein; GPx: Glutathione peroxidase; Ox-LDL: Oxidized low-density lipoprotein; ST: Skinfold thickness. One-way ANOVA test with linear contrast analysis were performed.
a
different from Q1.
b
different from all quartiles.