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. 2015 Jul 20;16(10):1486–1501. doi: 10.1080/15384047.2015.1071731

Figure 3.

Figure 3.

Western blot analysis showing that Bisindole PBD (5b) dampens the prosurvival PI3K/AKT/mTOR signaling pathway in breast cancer cells. (A, B) Proteins extracted from MCF-7 and MDA-MB-231 cells incubated with 4 μM of SAHA and 4, 8 μM of 5b for 24 h along with DMSO (0.1%) treated control cells were immunoblotted and hybridized with antibodies against AKT, p-AKT, PI3K and mTOR to check the level of expressions. β-Actin was used as gel loading control. The relative expression of proteins from 3 independent blots was plotted in histograms. Bars with * are significantly different from other groups p ≤ 0.01 except that they bear same symbol. In case of MDA-MB-231 histogram, bars with ** are significantly different from other groups p ≤ 0.01 and Bars with * are significantly different from other groups p ≤ 0.05 except that they bear same symbol. (C, D) Co- treatment with Q-VD-OPH 500 nm, SAHA 4 μM and 5b 4 μM was given to examine the expression pattern of AKT, p-AKT, PI3K and mTOR protein levels. β-Actin was used as loading control. Histograms represent data from 3 independent experiments. Data are represented as the mean ± SD. *P < 0.05.