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. 2015 Jul 20;16(10):1486–1501. doi: 10.1080/15384047.2015.1071731

Figure 6.

Figure 6.

Effect of 5b on post translational modifications of p53 and tubulin acetylation. (A, B) Proteins extracted from cells treated with indicated doses of Sirtinol, SAHA and 5b for 24 h were immunoblotted with antibody specific to acetylated tubulin, p53, acetylated p53(Lys373), acetylated p53(Lys382), phospho p53(Ser20), phospho p53(Ser15)and NF-kB. β-actin was taken as loading control. ‘*’ p < 0.05; bars with same symbols are dissimilar statistically. (C, D) Immunofluorescence studies with anti-acetylated tubulin of cells after treatment with 5b. Images were observed under confocal microscope (Olympus FV1000) and processed with flow view version 1.7c software program. Bar represents 10 µM scales. (E, F) Pretreated cells were subjected to protein gel blot to decipher the role of 5b upon HDAC6 expression. Relative density has been calculated taking β-actin as loading control. Bars represent mean + SD.