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. 2015 Jul 20;16(10):1486–1501. doi: 10.1080/15384047.2015.1071731

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Figure 9. — Methylation analysis at the p21 promoter by MSP in MCF-7 and MDA-MB-231 cells. (A) Genomic sequence of the p21 promoter (www.urogene.org/methprimer) revealed the presence of a high content of GC in CpG islands (blue). Methylated and unmethylated primers for p21 were desingned from Methprimer software. (B) Expression of the methylated and unmethylated DNA estimated by methylated and unmethylated primers obtainded from methprimer software. M primer anneal only to methylated sequences and U primer anneal unmethylated sequences. We took primer set 1 from the software output.