Figure 5.

Mobility of eGFP-H2B in somatic cell nuclear transferred (SCNT) embryos Mobility of eGFP-H2B was examined in the pseudopronucleus of SCNT embryos. 3T3 cells stably expressing eGFP-H2B were trypsinized and then used as donors to reconstruct SCNT embryos. At the same time, the remaining cells were seeded on glass-bottomed dishes. The SCNT embryos, control 1-cell stage embryos, and 3T3 cells were analyzed 10–13 h after being transferred, inseminated, and seeded, respectively. More than 4 independent experiments were performed and the data were accumulated. In total, more than 19 cells were examined in each group. Error bars indicate standard errors. The asterisks indicate statistical differences (P<0.05; by Student t-test)