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. 2015 Sep 29;16(11):1660–1670. doi: 10.1080/15384047.2015.1099769

Figure 7.

Figure 7.

Treatment of ovarian carcinoma cells with [celecoxib + sorafenib + sildenafil] reduces expression of ATAD3A, WASF3 and GST-p that is prevented by over-expression of GRP78 and HSP27. Upper: The basal expression of ATAD3A, GST-Pi, KISS1 and WASF3 was measured in Spiky, OVCAR, CAOV3 and PA-1 ovarian carcinoma cells by immuno-fluorescence in the Hermes Wiscan system at 10X magnification. Lower: Spiky tumor cells were transfected with an empty vector plasmid or plasmids to express HSP27 and GRP78 together. Twenty four h after transfection, cells were treated with vehicle or [sorafenib (2 μM) + sildenafil (2 μM) + celecoxib (2 μM)] for 6h. Six h after drug treatment, cells were fixed in place and immuo-staining performed to detect the expression of ATAD3A; GST-Pi; KISS1; and WASF3, using a Hermes WiScan wide field microscope at 10X.