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. 2016 Apr 26;11(4):e0154366. doi: 10.1371/journal.pone.0154366

Fig 9. General impact of IFITM overexpression in several infection parameters.

Fig 9

(A). Quantitation of ASFV viral DNA at 16 hpi in Vero-IFITM cells compared to empty controls. (B-C). Flow cytometry analysis showing percentages of infected Vero-IFITM cells at a MOI of 1 pfu/cell relative to empty controls evaluated by early p30 expression at 6 hpi (B) or late p72 protein expression at 16 hpi. (D-E). Viral protein expression in IFITM expressing cells. Vero-IFITMs and empty control cells were infected at a MOI of 1 pfu/cell. WB quantification of early p30 expression at 6 hpi (D) and late p72 expression at 16 hpi (E). (F). Western blot images of previous panels D and E. (A-E). Graphics depict mean±SD of densitometry values from three independent experiments. Statistically significance was evaluated by a one-way ANOVA followed by Bonferroni’s multiple comparison test. Differences are marked with asterisks as indicated (*p<0.05; **p<0.01; ***p<0.001).