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. 2016 Mar 30;15(6):827–839. doi: 10.1080/15384101.2016.1149273

Figure 5.

Figure 5.

Proliferation pattern of trophoblasts under hypoxia and depletion of BCL6 reduces proliferation. (A) HTR cells were cultured in an incubator supplied either with 21% O2 (normoxia) or with 3% O2 (hypoxia) for indicated time periods for the evaluation of cell viability. The results are presented as mean ± SD and statistically analyzed. ***p < 0.001, **p < 0.01, *p < 0.05. (B) HTR cells were treated as in (A) and harvested for Western blot analysis with indicated antibodies. β-actin served as loading control. (C) JAR cells were treated as in (A) for viability evaluation. The results are presented as mean ± SD and statistically analyzed. *p < 0.05. (D) Treated JAR cells were harvested for Western blot analysis with indicated antibodies. β-actin served as loading control. (E) BeWo cells were treated as in (A) for viability evaluation. The results are presented as mean ± SD and statistically analyzed. *p < 0.05, ***p < 0.001. (F) Treated BeWo cells were harvested for Western blot analysis with indicated antibodies. β-actin served as loading control. (G) HTR cells were non-treated, treated with control siRNA or siRNA against BCL6 and cultured for indicated time periods under hypoxia. The viability was measured and the results are presented as mean ± SD and statistically analyzed. *p < 0.05. (H) Cellular lysates were prepared from HTR cells for Western blot analysis with indicated antibodies. β-actin served as loading control. (I) JAR cells were non-treated, treated with control siRNA or siRNA against BCL6 and cultured for indicated time periods under hypoxia. The viability was measured and the results are presented as mean ± SD and statistically analyzed. **p < 0.01. (J) Cellular lysates were prepared from JAR cells for Western blot analyses with indicated antibodies. β-actin served as loading control. (K) BeWo cells were non-treated, treated with control siRNA or siRNA against BCL6 and further cultured for indicated time periods under hypoxia. The viability was measured and the results are presented as mean ± SD. (L) Cellular lysates were prepared from BeWo cells for Western blot analysis with indicated antibodies. β-actin served as loading control.