Figure 6.

BCL6 is stabilized and its suppression induces apoptosis under stress situation. (A) Western blot analysis. HTR cells were subjected to 25 µM CHX (left panel), 50 µM H₂O₂ (middle panel) or both (right panel), for indicated time periods and harvested for Western blot analysis with indicated antibodies. β-actin served as loading control. (B) Quantification of BCL6 in (A), relative to corresponding β-actin. The dot lines indicate the half-life time of corresponding BCL6. (C) HTR cells were non-transfected as control (con), transfected with control siRNA (si con) or siRNA against BCL6 (si BCL6) for 24 h and subjected to increasing concentrations of H₂O₂ for further 12 h. Cells were then harvested for Western blot analysis with BCL6 antibody. β-actin served as loading control. (D) Relative activity of caspase-3/7. The value of non-transfected and non-treated cells was defined as 1-fold. ***p < 0.001, **p < 0.01.