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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Bioorg Med Chem. 2016 Jan 6;24(5):938–956. doi: 10.1016/j.bmc.2016.01.007

Figure 3.

Figure 3

Cleavage of the glycine conjugates of 2' CA4-amine 16 and the amino dihydronaphthalene 44 by LAP. (A) Prodrug 16 was treated with 0.5 units of LAP for 40 h. A single peak corresponding to the product 2' CA4 amine 7 (tR = 31.29 min) was observed (HPLC chromatogram mobile phase: 26% acetonitrile/74% water containing 0.05% TFA). Inset: Control, compound 16 was incubated for 40 h without LAP. (B) Rate study for the cleavage of prodrug 16 to form 2' CA4 amine 7. Inset: Calibration curve for 2' CA4 monoamine 7. (C) Prodrug 44 was treated with 0.05 units of LAP for 2 h. A single peak corresponding to the product amino dihydronaphthalene 32 (tR = 12.10 min) was observed (HPLC chromatogram mobile phase: 28% acetonitrile/72% water containing 0.05% TFA). Inset: Control, compound 44 was incubated for 2 h without LAP. (D) Rate study for the cleavage of prodrug 44 to form amino dihydronaphthalene 32. Inset: Calibration curve for amino dihydronaphthalene 32