Figure 8. Old Ndrg1-deficient T cells aggravate autoimmunity.

(a) CD4⁺ T cells isolated from more than 1-year-old wild-type or Ndrg1-knockout mice were adoptively transferred to Rag1^−/− mice. EAE was induced and the mean clinical score was measured as described in Methods. Results are representative of two independent experiments; error bars, s.e.m. (b,c) Experimental autoimmune uveitis (EAU) was induced in 1- to 1.5-year-old wild-type or Ndrg1-knockout mice by injecting them with uveitogenic peptides. The eyes were haematoxylin and eosin stained (b) and disease severity was scored histopathologically (c). Representative diseased eyes are shown in b. The wild-type eye shows focal retinal folds and the knockout eye shows diffuse retinal folds/detachments, vasculitis and granuloma formation. Pooled data from ten independent experiments are shown in c. Incidences of diseased eye among total eyes are indicated in parenthesis. P-values, Mann–Whitney U-test.