Fig 5. Arginine deprivation response is mediated by MPK2.

Promastigotes of L. mexicana mutants lacking both copies of the MPK2 gene (Δmpk2) and those containing a single wild type copy of the gene re-inserted into their genome (addback) were subjected to 2 hours arginine deprivation as described in Fig 1. A) Northern analysis of total mRNA from these cells using LmxMPK2 probe. B) Arginine transport was measured for Δlmxmpk2 (left panel) and addback (right panel) cells grown in the presence (♦) or absence (•) of arginine.