Fig. 6.

MDA5 ectopic expression sustains RIG-I-induced active IRF-3 levels. A549 cells were transfected with either empty, Flag-RIG-I- or Flag-MDA5-encoding plasmids before being transfected with 5′ppp-dsRNA for the indicated times. WCE were analyzed by immunoblot (IB) using anti-Flag and anti-actin antibodies to control the expression of Flag-tagged proteins. IRF-3 dimerization was analyzed by native PAGE followed by immunoblot using anti-IRF-3 antibodies. D = Dimer; M = monomer. The data are representative of three different experiments with similar results.