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. 2016 Apr 8;5:e14294. doi: 10.7554/eLife.14294

Table 1.

Statistics from crystallographic analysis.

DOI: http://dx.doi.org/10.7554/eLife.14294.010

RecJ-dTMP RecJd-DNA RecJd-DNA-SSBct
Complex I Complex II Complex III
Data collection
Space group P 3221 P 3221 P 3221
Cell dimensions a, b, c (Å) 106.53 105.83 102.22
106.53 105.82 102.22
161.90 165.40 166.12
Wavelength (Å) 0.9792 0.9792 0.9792
Resolution (Å) 30–2.7 (2.77–2.70) 30–2.6 (2.66–2.60) 30–2.3 (2.35–2.30)
R-meas 5.5 (64.1) 6.5 (79.1) 7.4 (63.7)
I/σI 27.0 (4.0) 22.6 (3.0) 17.0 (2.9)
Completeness (%) 98.9 (99.5) 99.6 (99.4) 99.4 (94.1)
Redundancy 8.9 8.3 7.2
Refinement
Resolution (Å) 30–2.7 30–2.6 30–2.3
No. reflections 29747 33594 45296
Rwork/Rfree 20.61/25.20 18.47/22.84 22.56/23.89
No. atoms
Protein/DNA 5262/- 5373/286 5342/182
Ligand/Ion 21/2 35/2 55/2
Waters 12 - 142
B factors
Protein/DNA 79.8/- 70.4/103.9 58.2/93.7
Ligand/Ion 66.7/76.6 99.8/68.2 74.5/61.9
Water 62.7 - 48.9
Rmsd
Bond length (Å) 0.012 0.005 0.012
Bond Angle (°) 1.085 0.849 1.464
Ramachandran statitics
Favored (%) 98.0
0
99.1 98.9
Allowed (%) 2.0 0.9 1.1
Outliers (%) 0 0 0

Values in parentheses refer to the highest resolution shell.

R factor = Σ||F(obs)- F(calc)||/Σ|F(obs)|.

Rfree = R factor calculated using 5.0% of the reflection data randomly chosen and omitted from the start of refinement.

RecJd denotes catalytic inactive drRecJ (H160A).