Extended Data Figure 3. CNC gives rise to neurons enriched in autonomic lineage.
a-c) Representative immunofluorescence images for expression of 5-HT (Serotonin), TUJ1 and TH in CNC-derived neurons. In contrast to ENC-derived lineages no serotonergic (5HT+) neurons were detected under cranial conditions despite the presence of many Tuj1+ neurons and increased percentages of TH+ cells. d,e) Flow Cytometry for TRKB and TRKC under CNC and ENC conditions. n=3 independent experiments. Scale bars= 50 μm.