Table 2. Kinetic parameters of CLLs toward different substrates.
Each data set represents the mean ± SE from triplicate measurements.
Enzyme | Substrate | Km (μM) | Vmax (nkat mg protein−1) | Vmax /Km |
SbCLL-1 | Cinnamic acid | 5300 ± 712 | 180 ± 16 | 0.03 |
4-Coumaric acid | 45 ± 3 | 536 ± 8 | 11.88 | |
Caffeic acid | 18 ± 2 | 190 ± 9 | 10.58 | |
SbCLL-5 | Cinnamic acid | 3060 ± 331 | 279 ± 23 | 0.09 |
4-Coumaric acid | 31 ± 3 | 687 ± 35 | 22.15 | |
Caffeic acid | 260 ± 15 | 600 ± 10 | 2.31 | |
SbCLL-7 | Cinnamic acid | 172 ± 17 | 168 ± 7 | 0.97 |
4-Coumaric acid | — | — | — | |
Caffeic acid | — | — | — |