Skip to main content
. Author manuscript; available in PMC: 2016 Aug 29.
Published in final edited form as: Nat Neurosci. 2016 Feb 29;19(5):678–689. doi: 10.1038/nn.4258

Figure 5. Chd7 controls the core myelinogenic regulatory network.

Figure 5

(a) Volcano plots depict gene expression changes between control and Chd7cKO spinal cord at P8. Significantly differential transcripts were highlighted in color and totaled in each direction (FDR < 0.05).

(b) Heat map representing the expression of OL differentiation-related genes in control and Chd7cKO spinal cords from two independent animals.

(c) qRT-PCR analysis of myelination-associated genes in P8 control and Chd7cKO spinal cords (The data are presented as mean ± s.e.m. n = 3 controls and 3 mutant tissues; for Mbp, *** p = 0.0008, t = 9.11; for Plp1 * p = 0.028, t = 3.37; for Cnp, *** p = 0.0001, t = 14.63; for Sox10, *** p = 0.0005, t = 10.05; for Tcf7l2, ** p = 0.0049, t = 5.62; for Myrf, * p = 0.03, t = 3.29; for Pdgfrα, p = 0.168, t = 1.68; for Hes1, * p = 0.027, t = 3.38; for Hes5, p = 0.98, t = 0.02; for Id2, * p = 0.049, t = 2.81; for Id4, * p = 0.03, t = 3.29; Two-tailed unpaired Student’s t test).

(d) The gene ontology (GO) analysis of the significantly down-regulated genes between control and Chd7cKO.

(e) Protein-protein interaction networks among down-regulated genes in Chd7cKO are plotted using a ToppGene suite.

(f,g) qRT-PCR validation of selected genes involved in lipid metabolism (f) and cytoskeleton organization in control vs. Chd7cKO spinal cords at P8 (g) (The data are presented as mean ± s.e.m. n = 3 controls and 3 mutant tissues; for Enpp2, ** p = 0.002, t = 6.67; for Nfya, ** p = 0.0018, t = 7.41, for Pik3r3, * p = 0.046, t = 2.86; for Elovl7, ** p = 0.0012, t = 8.22; for Gsn, *** p < 0.001, t = 14.809; for Ermn, *** p < 0.001, t = 8.659; for Mtap7, *** p < 0.001, t = 10.181; for Tppp, *** p < 0.001, t = 9.907; Two-tailed unpaired Student’s t test).