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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Transplantation. 2016 May;100(5):1015–1021. doi: 10.1097/TP.0000000000001141

Figure 4. Mice in HFD develop increased alloreactivity to in vivo DST immunization.

Figure 4

C57BL/6 mice (H-2b) in LFD and HFD were immunized with allogeneic BALB/c splenocytes (106 cells) subcutaneously under the skin of the neck and cells isolated from cervical draining lymph nodes were analyzed 14 days later by ELISpot for their IFNγ production upon in vitro restimulation with irradiated syngeneic or donor splenocytes. The data (mean +/− SEM of 3 mice/group) are representative of 2 independent experiments with at least 3 mice/group. Comparisons were performed by one-way ANOVA. *: p <0.05.