Fig. 3. Effect of IPA-3 and Pir 3,5 on the ability of physiological (0.3 nM) and supraphysiological (100 nM) concentrations of CCK and TPA (1μM) to stimulate various adapter proteins (paxillin and p130^CAS).

Rat pancreatic acinar cells were processed as stated in Figure 2. Membranes were analyzed using anti-pY118 paxillin and anti-pY410 p130^CAS. Antibodies detecting total amount of these kinases were used to verify loading of equal amounts of protein. The bands were visualized using chemoluminescence and quantification of phosphorylation was assessed using scanning densitometry. Both a representative experiment of 4 others and the means of all the experiments are shown. * P< 0.05 vs. control, # P< 0.05 vs. IPA-3 alone, ∞ P< 0.05 vs. Pir 3,5 alone and $ P< 0.05 comparing stimulants (CCK or TPA) preincubated with 1% DMSO vs. stimulants pre-incubated with IPA-3 or Pir 3,5, respectively.