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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Feb 18;1862(6):1122–1136. doi: 10.1016/j.bbadis.2016.02.008

Fig. 7. Effect of the PAK2 inhibitor, IPA-3 and its inactive control, Pir 3,5 on the ability of physiological (0.3 nM) and supraphysiological concentrations of CCK (100 nM) and TPA to stimulate the PKC pathway (PKD, Marcks), PTEN, p38, Src and GSK-3β.

Fig. 7

Rat pancreatic acinar cells were processed as stated in Figure 2. Membranes were analyzed using anti-pS9 GSK-3-β, anti-p-S380 PTEN, anti-pS744/748 PKD, pS152/156 Marcks, anti-pY183 p38 and anti-pY416 Src. Antibodies detecting total amount of these kinases or tubulin were used to verify loading of equal amounts of protein. These results of the experiments shown are representative of 4 others.