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Purification of l-asparaginase from Bacillus subtilis KDPS1
| Steps for purification | Total protein (mg) | Total activity (U/ml) | Specific activity (U/mg) | Fold purity | % yield |
|---|---|---|---|---|---|
| Crude enzyme | 5.85 ± 3.34 | 46.87 ± 7.12 | 8.01 ± 3.62 | 1.00 | 100 |
| Ammonium sulphate precipitation | 1.12 ± 2.64 | 41.10 ± 6.15 | 36.69 ± 7.44 | 4.58 | 87.61 |
| Ion exchange chromatography | 0.41 ± 1.12 | 39.79 ± 4.71 | 97.04 ± 12.28 | 12.11 | 84.89 |
