Fig. 2.

Enhanced neuronal gene expression in F11 cells treated with miR-124a. a F11 cells were transfected with miR-124a or miR-scramble oligomer, respectively. Morphological changes were observed at 24 and 48 h after transfection. MiR-124a-transfected cells showed significant neurite outgrowth, compared to non-transfected or miR-scramble-transfected cells. Scale bars, 100 μm. b F11 cells transfected with either miR-124a or miR-scramble were fixed using 4 % paraformaldehyde for 20 min. Antibodies against enolase, Tuj1, NF, and GFAP were used to evaluate the neuronal differentiation stage. Tuj1 and NF expression increased significantly in miR-124a-transfected F11 cells