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A 500 ml spinner batch culture of CHOK1SV cells. Samples were taken in triplicate every 24 h over a period of 168 h and viable cells as well as percentage viability were determined by hemocytometer counts in conjunction with the TB exclusion assay. The cells reached a maximum cell density of 5.74 × 106 cells/ml with a viability of 95.67 ± 0.58 % at 144 h
