Fig. 4.

Online flow cytometry to measure apoptosis versus manual flow cytometry measurements via dual Annexin-V FITC and PI staining on CHOK1SV cultured cells grown in a 500 ml stirred vessel. The plots show online readings for the percentages of viable (blank triangles), early apoptotic (blank squares), and late apoptotic plus necrotic cells (blank circles) compared to the manual readings for the percentages of viable (black filled triangles), early apoptotic (black filled squares), and late apoptotic plus necrotic cells (black filled circles)