Table 1.

Programmed command sequence for apoptosis staining procedure in the FCP system, SL = sample loop as described in Kuystermans et al. (2012)

Steps	Command	Stream/port	Flow (ml/min)	Volume (μl/ml)	Time (s)
1	Draw sample	1	4	1,000	–	These steps are for removing the dead volume from the tubings
2	Incubate	–			30
3	Test trigger	–	–		–
4	Inject SL	–	3	1,000	–
5	Draw sample	1	10	1,000	–
6	Incubate	–	–	–	10
7	Inject SL	–	5	1,000	–
8	Test trigger	–	–	–	–
9	Wash	–			–
10	Draw sample	1	4	900	0
11	Incubate	–	–	–	30
12	Test trigger	–	–	–	–
13	Incubate	–			50	Apoptosis sample preparations steps
14	Draw sample	1	5	800	–
15	Draw stain (Annexin V)	6	5	200	–
16	Draw sample (air)	2	5	500	–
17	Inject SL	–	4	500	–
18	Incubate	–	–	–	600
19	Draw stain (PI)	7	5	10	–
20	Draw sample (air)	2	5	500	–
21	Inject SL	–	4	500	–
22	Incubate	–	–	–	30
23	Default	–	–	–	–
24	Incubate	–	–	–	250
25	Test trigger	–	–	–	–	Washing and incubation steps
26	Incubate	–	–	–	50
27	Test trigger	–	–	–
28	Incubate	–	–	–	50
29	Wash	–	10	2,000	–
30	Incubate	–	–	–	3,600
31	Incubate	–	–	–	3,600
32	Incubate	–	–	–	3,600
33	Incubate	–	–	–	3,600
34	Test trigger	–	–	–	–
35	Incubate	–	–	–	3,600