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Plasma membrane stability of buffalo sperm evaluated under fluorescent microscope using Annexin-V/PI assay. a Apoptotic sperm (labeled Annexin-V only, green fluorescence), b Necrotic sperm (labeled only PI, red fluorescence). c Early necrotic sperm (labeled both Annexin-V and PI, green and red fluorescence), and d viable sperm (not labeled, no fluorescence)
