Table 5.
Influence of co-expression of the receptors on cytotoxicity of activated Cry1Ac to Sl-HP cells
| Transfected Sl-HP cells | Probit regression analysis | R | EC50 ± SE (µg/ml) |
|---|---|---|---|
| GFP* | y = 3.982 + 1.446x | 0.97 | 5.08 ± 0.42a# |
| Cad-mCherry* | y = 6.825 + 1.498x | 0.97 | 0.060 ± 0.008b |
| Cad-mCherry + APN1-GFP-GPI** | y = 8.849 + 2.922x | 0.98 | 0.048 ± 0.011b |
| Cad-mCherry + ALP2-GFP-GPI** | y = 9.390 + 2.871x | 0.98 | 0.030 ± 0.014b |
| Cad-GFP* | y = 7.124 + 1.847x | 0.98 | 0.070 ± 0.020b |
| Cad-GFP + APN1* | y = 8.027 + 2.451x | 0.96 | 0.058 ± 0.006b |
| Cad-GFP + ALP2* | y = 8.038 + 2.656x | 0.98 | 0.072 ± 0.012b |
* The cells emitting fluorescence were counted for the calculation of EC50
** The cells emitting both green and red fluorescence were counted for the calculation of EC50, and more than 80 % of the transfected cells
#The different letters represented significant differences at p < 0.05