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. 2016 Apr 27;36(17):4802–4815. doi: 10.1523/JNEUROSCI.3186-15.2016

Figure 6.

Figure 6.

Genetic deletion of Epac2 blocked the increase in the AMPAR/NMDAR ratio induced by a single cocaine injection in vivo. Wild-type control and Epac2−/− mice received a single intraperitoneal injection of saline or cocaine (15 mg/kg). Midbrain slices were prepared from these four groups of mice the next day. A, Representative AMPAR- and NMDAR-mediated evoked EPSCs recorded from VTA dopamine neurons. B, A single cocaine injection in vivo significantly increased the AMPAR/NMDAR ratio in Epac+/+ slices (***p < 0.001; n = 8 for each group), although this increase was blocked in Epac2−/− slices (***p < 0.001; n = 8 for each group). C, Representative mEPSCs recorded from VTA dopamine neurons in saline- or cocaine-treated Epac+/+ and Epac2−/− mice. D, E, A single cocaine injection in vivo significantly increased the frequency (***p < 0.001) and amplitude (***p < 0.001; n = 9–10) of mEPSCs in Epac+/+ slices, although these increases were blocked in Epac2−/− slices (both ***p < 0.001; n = 9–10).