Table 1. Most abundantly expressed miRNAs in PRRSV infection and matched normal lung tissues.

miR_name	miR_seq	TPM in normal lung			TPM in infected lung			Normal/infection
		Sample 1	Sample 2	Sample 3	Sample 1	Sample 2	Sample 3
ssc-miR-30d_R-1	TGTAAACATCCCCGACTGGAAGC	3345	3454	3521	1039	1004	996	3.40
ssc-miR-27b	TTCACAGTGGCTAAGTTCTGC	21271	23567	22453	9807	10002	10244	2.24
ssc-miR-145_R-1	GTCCAGTTTTCCCAGGAATCCCT	5619	5746	5531	2836	3021	2968	1.91
ssc-miR-23a_R + 1	ATCACATTGCCAGGGATTTCCA	2447	2563	2451	1357	1532	1520	1.69
ssc-miR-199a-5p	CCCAGTGTTCAGACTACCTGTTC	3755	3852	3732	2322	2531	2438	1.56
ssc-miR-99a_R-1	AACCCGTAGATCCGATCTTGT	1625	1707	1797	1161	1066	1089	1.55
ssc-miR-199b_R + 1	CCCAGTGTTTAGACTATCTGTTC	1022	918	993	487	401	454	2.19
ssc-miR-133a-3p_R + 1	TTGGTCCCCTTCAACCAGCTGT	832	632	682	387	309	325	2.1
ssc-miR-423-3p	AGCTCGGTCTGAGGCCCCTCAGT	643	549	742	328	303	287	2.1
ssc-miR-374b-5p	ATATAATACAACCTGCTAAGTG	299	277	214	165	119	138	1.87
ssc-miR-532-5p	CATGCCTTGAGTGTAGGACCGT	558	494	662	311	308	285	1.90
hsa-miR-181a*	ACCATCGACCGTTGATTGTACC	228	185	208	105	84	69	2.41
hsa-miR-148a*	AAAGTTCTGAGACACTCCGACT	362	282	212	209	190	198	1.43
hsa-miR-221*_R + 4	ACCTGGCATACAATGTAGATTTCTGT	99	112	94	213	181	157	1.36
ssc-miR-221	AGCTACATTGTCTGCTGGGTTT	105	125	111	257	204	217	1.99
hsa-miR-34c-3p_L-1	ATCACTAACCACACGGCCAGG	86	61	72	187	161	193	2.47
PN-ssc-miR-1307-5p	TCGACCGGACCTCGACCGGCT	224	187	155	407	383	353	2.02
PN-ssc-miR-24_R-3	TGGCTCAGTTCAGCAGGAA	181	142	111	396	353	341	2.51
hsa-miR-449a	TGGCAGTGTATTGTTAGCTGGT	292	281	201	658	622	617	2.45

miRNAs with TPM >100 and more than a 1.5-fold alteration are listed, but miRNAs with TPM > 1000 and more than a 1.5-fold alteration were considered most likely to be important in PRRSV pathogenesis in this study.