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. 2016 Apr 27;6:24558. doi: 10.1038/srep24558

Figure 2. The morphology change of SMMC7721cells during the migration and matrigel invasive assay.

Figure 2

(a,b) Migration assay and matrigel invasive assay. When treated with M2CM, the morphology of SMMC7721 cells was found to have changed into a diffused fibroblast-like morphology characteristic of EMT, this was not observed with the remaining groups; (c) Changes in the mRNA expressions of the EMT-related genes in SMMC7721 cells after treated with UM0CM, M1CM and M2CM. A down-regulation of E-cadherin (E-cad) and up-regulation of N-cadherin (N-cad), vimentin (Vim) and α-smooth muscle actin (α-SMA) were found after M2CM stimulation. *p < 0.05.