Table 1.
Bacterial strains, plasmids, and phage used in this study.
Strain, plasmid, or phage | Relevant characteristics | References or source |
---|---|---|
STRAINS | ||
JS | S. enterica Serovar Typhimurium CVCC541 | Supplied by China Institute of Veterinary Drug Control |
JSΔcpxR | Derivative of JS that lacks cpxR | Huang et al., 2015 |
JSΔacrB | Derivative of JS that lacks acrB | Huang et al., 2016 |
JSΔacrBΔcpxR | Derivative of JS that lacks both cpxR and acrB, ΔcpxR::kan | Huang et al., 2016 |
SH(1–9) | Clinical isolates from chicken in Henan province in China | This study |
SH(1–9) ΔcpxR | Derivative of SH(1–9) that lack cpxR, ΔcpxR::kan | This study |
PLASMIDS | ||
pKD4 | Gene knowout help vector: repR6Kγ ApR FRT KmR FRT | From E. coli Genetic Stock Center in Yale University |
pKD46 | Gene knowout help vector: ApR ParaBAD γ β exo | |
pBAD | Expression vector: reppBR322ApR araC PBAD | Invitrogen Corporation |
pBAD-CpxR | cpxR gene cloned to pBAD; ApR | This study |
pBAD-CpxR* | Mutation sequence CpxR* cloned to pBAD; ApR | This study |
PHAGE | ||
P22HT105/int | Transduction medium of Salmonella | Supplied by Microbial Genomics Research Center of Harbin Medical University |