Table 2.
Function | Primer | Sequence (5′ → 3′) | References or source |
---|---|---|---|
Amplification of cpxR gene | XholI-cpxR-F HindIII-cpxR-R | CGCTCGAGATGAATAAAATCCTGTTAGT GCAAGCTTTCATGAAGCGGAAACCATCA | This study |
Preparation of cpxR* | XholI-cpxR-F Rm | CGCTCGAGATGAATAAAATCCTGTTAGT ACTTTTGCTTGCCGTCATGATGCCGAAG | This study |
Fm Hind III-cpxR-R | CTTCGGCATCATGACGGCAAGCAAAAGT GCAAGCTTTCATGAAGCGGAAACCATCA | This study | |
REAL-TIME RELATIVE QUANTITATIVE PCR | |||
ompF | ompF-F ompF-R | CCTGGCAGCGGTGATCC AAATTTCTGCTGCGTTTGCG | Tatavarthy and Cannons, 2010 |
ompC | ompC-F ompC-R | TCGCAGCCTGCTGAACCAGAAC ACGGGTTGCGTTATAGGTCTGAG | Hu et al., 2011 |
ompD | ompD-F ompD-R | GCAACCGTACTGAAAGCCAGGG GCCAAAGAAGTCAGTGTTACGGT | Hu et al., 2011 |
ompW | ompW-F ompW-R | CAGCAGCAAAGTGCGTCCTTATGT AGACAGAGGCGCCAATTAACCAGT | Hu et al., 2011 |
stm3031 | stm3031-F stm3031-R | TGCAAGCAGGGAGTAATAACGGGT TCACTTGGATACGCCCAGTCCCAT | Hu et al., 2011 |
stm1530 | stm1530-F stm1530-R | CGTCTCGGTTTTGCTGGTTTGG GCCGTCATTTTTACCCTGATACTGC | Hu et al., 2011 |
acrB | acrB-F acrB-R | CGTGAGCGTTGAGAAGTCCT GGCGTCAGTTGGTATTTGGT | Li et al., 2009 |
acrD | acrD-F acrD-R | TCCGGCCAAATTGAATAGTT TCGGAACCGTCCTGATTAAC | Eaves et al., 2004 |
acrF | acrF-F acrF-R | TATCTGGCTGGATGCGAATCTGCT ACTTTGCCGAACTCTTCCGGATCT | Eaves et al., 2004 |
mdtA | mdtA-F mdtA-R | GAATGCGCGTCGTGATCTG TCCAGTTCCTGACGGGAAAC | Nishino et al., 2007 |
marA | marA-F marA-R | ATACATCCGCAGCCGTAAAA GTGATTCGCCATGCATATTG | Li et al., 2009 |
soxS | soxS-F soxS-R | TACGGTAACGCATCAAACA ACAGGCGGTGACGGTAAT | Li et al., 2009 |
16SrRNA | 16SrRNA-F 16SrRNA-R | TTAGATACCCTGGTAGTCCACGC TTGCGGGACTTAACCCAAC | Li et al., 2009 |
The underlined bases are restriction sites.