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. 2016 Apr 27;6:25130. doi: 10.1038/srep25130

Figure 1. Inhibition of TNF-α and IL-6 production as indicated by chalcone derivatives.

Figure 1

After plating and 24 h of growth, cells were pretreated for 2 h with the indicated concentrations of each chalcone derivative before stimulated with LPS (0.5 μg/mL) for 22 h. Culture medium and proteins were then collected. The TNF-α and IL-6 levels in the medium were determined by ELISA. Data are expressed as fold change relative to control values (samples treated with LPS alone), mean ± SEM. n ≥ 3. *p < 0.05 and **p < 0.01 vs. only-LPS stimulated group.