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. 2016 Apr 27;6:25130. doi: 10.1038/srep25130

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Copyright © 2016, Macmillan Publishers Limited

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(A) Molecular docking of compound 20 with MD2 protein was carried out with the Sybyl-2.0 molecular modeling software from Tripos. Compound 20 did not inhibit biotin-LPS binding to (B) rhMD2^R90A/Y102A, (C) rhMD2^R90A and (D) rhMD2^Y102A determined by ELISA. SPR analysis showed no interaction between compound 20 and (E) rhMD2^R90A/Y102A, (F) rhMD2^R90A and (G) rhMD2^Y102A respectively. Data are mean values (±SEM) of at least three separate experiments.