Skip to main content
. 2016 Mar 23;8(4):39. doi: 10.3390/cancers8040039

Figure 1.

Figure 1

(A) HEK-293 cells transfected with expression constructs for MDM2 paired with an empty vector or a construct expressing NORE1A. MDM2 expression can be easily detected when co-expressed with an empty vector. Levels of MDM2 are suppressed when co-expressed with NORE1A; (B) HEK-293 cells were transfected with expression plasmids expressing NORE1A paired with either an empty vector or a construct expressing MDM2. 24 h post-transfection, the two groups were split and allowed to re-adhere to the culture dishes. Then the matched sets were treated with either DMSO (carrier) or MG132 at a final concentration of 10 μM for 12 h. The cells were lysed and examined on Western blot. Here we show that inhibition of the proteasome disrupts the ability of NORE1A to suppress MDM2, suggesting that indeed NORE1A is controlling MDM2 protein levels via the 26S proteasome. Representative blots of 2 independent experiments are shown in both panels.