Table 5.

Detail of locus, primer sequence, Tm, Motif, Alleles size, percentage polymorphism, No. of alleles, and PIC value of different primers used to evaluate genetic diversity within Trichoderma isolates.

	Forward (5′-3′)	Reverse (5′-3′)	Tm °C	Motif	Allele size	% Poly-morphism	No. of alleles	PIC
Ta 1195	attgacgactcatagcgttt	ttttcagtcaggtctcgttt	54.9	(agac)9	300–320	100	2	0.95
Ta 1394	gccttacttgaagccactaa	ctagcttgcctgcttatgat	55.0	(ga)8-(at)6	200–500	100	2	0.97
Ta 1644	gacgtctcgaatcttcaatc	atgtgacaattgaacagcaa	54.8	(tga)11	250	100	1	0.37
Ta 2172	atgtacagtagcaccgctct	ttgtttgctttctggacttt	55.1	(gca)15	350–600	100	4	0.67
Ta 2383	accaccagcgcattct	gttggttcgtctgagatagc	55.5	(ca)20	350	100	1	0.71
Ta 2584	agctcgatgtctatagcagg	tattgagctcatccttggtt	54.8	(ctg)9	350–400	100	2	0.79
Th79	gtacagcagcaattcgtaca	cactgatggtgcatattgag	54.9	(aca)21	150–500	100	5	0.15
Th109	tgcatctctccagattttct	tccagaactccatccataac	54.9	(ct)9	200–400	100	2	0.96
Th276	ctaagcctacggacaagaag	gttgccgttgttgttattg	55.0	(aac)29	375–600	100	4	0.44
Th697	gctggcattaggtacagaac	ttagtcaaggctttttgcat	55.1	(aca)12	275–300	100	2	0.71
Th1583	caggtactattcgtaggcgt	ttctgaggttgaaggtgact	54.6	(tct)14	200–500	100	4	0.75
Th2067	tggcaatgtctctctctttt	ttttgcttctcttcctcttg	55.0	(tc)16	120–900	100	5	0.69