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. 2016 Apr 27;6:25222. doi: 10.1038/srep25222

Figure 2. SCID phenotype of RAG2/IL2RG deficient pigs at 34 days of age.

Figure 2

(a) Representative images showing thoracic thymus (black arrows) and cervical thymus (blue arrow) in WT pigs, while thoracic and cervical thymus were lacking in some RAG2/IL2RG pigs. (b) Representative images of the mesentery showing the lack of MLN in some RAG2/IL2RG pigs. Representative flow cytometry of MNC from ileum (c) and blood (d) showing a significant reduction of B cells (CD79+), T cells (CD3+), and NK cells (CD3−CD16+), as indicated by dot plots gated within lymphocytes. Cy7, cyanine 7; APC, allophycocyanin; PE, phycoerythrin. (e) Total number of MNC from 40 cm ileum and 70 ml blood were isolated and quantified for WT (n = 4) and RAG2/IL2RG deficient pigs (n = 6). (f) Lymphocytes in ileum and blood were quantified based on the total number of MNC and the proportion of cells within MNC (Supplementary Fig. 5). Data are presented as means ± s.e.m. with individual animal data points (e,f). Statistical significance was determined by Mann-Whitney test. NS, not significant, **P < 0.01.