Figure 5.

UDE increased ex vivo nodule formation in BMSC derived from L5 of OVX rats. BMSC harvested from L5 (A) and proximal femur (B) from the groups indicated, and cultured in osteogenic medium to induce mineralization (see Materials and Methods). Left panels represent photomicrographs of mineralized nodules stained with alizarin red-S. Right panels, stain was extracted and quantified colorimetrically. All values are expressed as mean ± SEM (n = 6 rats/group); ***p<0.001 compared with sham (vehicle treated).