Figure 2.
Generation of iPSCs from hPMSCs. A. hPMSCs were transfected with episomal plasmid vectors for pluripotent stem cell induction. After 20 days, the typical ES-like colonies emerged. B. The hPMSC-iPSC colonies at passage 30 were maintained on vitronetion in chemical defined medium. C. The hPMSC-iPSC colonies were stained positive for AP. D. The hPMSCs were stained negative for AP. Images were acquired at 200X magnification. Representative images are shown. E. Growth curves of ESCs, hPMSC-iPSCs and hPMSCs.