Figure 5.

Chondrogenic differentiation of CD105+/CD166+ cartilage-derived mesenchymal stem cells (CMSCs) in normal (a) and degraded cartilage (b). Chondrogenic differentiation cells are induced using chondrogenic induction medium (right image ×200), control cells are cultured in MSC growth medium (left image ×200), and immunostained for (A) Aggrecan (B) Collagen 2 (C) Sox-9 after 28 days of induction (n=3). (D) The expressions of Collagen 2 and Aggrecan mRNA are analyzed by RT-PCR (n=3), no statistical differences are shown between degraded and normal group after chondrogenic differentiation, #P<0.05 compared with control cells from the same group. Data are presented as the mean ± SEM.