Figure 2.

The anti-fibrotic effect of propofol is associated with suppression of TGF-β/Smad signaling in early UUO mousekidney. A. Immunohistochemical analysis of TGF-β1 (n = 5 per group). Scale bar: 50 μm. B, C. Western blot analysis of TGF-β1, iNOS, and p-Smad3. Representative blots from four independent experiments are shown. D-F. Densitometric analysis of protein expression relative to β-actin levels (n = 4 per group). G. qPCR analysis of TGF-β1 in the kidneys (n = 5 mice per group). H. Analysis of NO levels in the kidneys (n = 5 per group). Data are the means ± SEM.