Skip to main content
. 2016 Feb 15;8(2):460–472.

Figure 5.

Figure 5

Propofol ameliorates kidney fibrosis and suppresses EMT by regulating miR-155 in vivo and in vitro. A. Kidney miR-155 levels were measured using semi-quantitative real-time RT-PCR (n = 5 per group). B. MiR-155 levels were analyzed in control HK-2 cells and HK-2 cells treated with propofol with or without TGF-β1. C. Western blot analysis of cell lysis. HK-2 cells were transfected with mimic-miR155 with or without propofol; E-cadherin, SM-22, and iNOS levels were analyzed. D-F. Densitometric analysis of E-cadherin, SM-22α, and iNOS normalized to β-actin (n = 4 per group). Data are the means ± SEM. G. Western blot analysis of cell lysis. HK-2 cells were transfected with antagomir-miR155 with or without TGF-β1; E-cadherin, SM-22α, and iNOS levels were analyzed. H-J. Densitometric analysis of E-cadherin, SM-22α, and iNOS normalized to β-actin (n = 4 per group). Data are the means ± SEM.