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EPI pretreatment increases IFN-γ and TNF-α secretion by NK cells. MCF-7, SKBR-3, and MDA-MB-231 breast cancer cells were pretreated with EPI for 12 h before adding expanded human NK cells for 24 h. IFN-γ and TNF-α levels in the supernatant were detected by ELISA. The supernatant of NK cells without or with untreated cancer cells served as controls. Experiments were performed in triplicate; data are expressed as mean ± SEM. *P < 0.05, ***P < 0.01.
