Figure 6.

EPI pretreatment induces upregulation of Fas receptor on breast cancer cells and FasL on expanded NK cells. A. MCF-7, SKBR-3, and MDA-MB-231 breast cancer cells were pretreated with EPI for 12 h, washed, and cultured for 24 h. Untreated cells served as a control. Fas receptor expression by cancer cells was detected by flow cytometry. B. MCF-7, SKBR-3, and MDA-MB-231 cells were pretreated with EPI for 12 h before adding expanded NK cells for 24 h. FasL expression by NK cells was detected by flow cytometry. Mean fluorescence intensity is shown. Experiments were performed in triplicate; data are expressed as means ± SEM. ***P < 0.01.