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. 2016 Feb 15;8(2):506–515.

Figure 4.

Figure 4

miR-541 targets IRF7 in VSMCs. A. Schematic representation of IRF7 3’UTR showing the putative miR-541 target site. B. Relative luciferase activity of the indicated IRF7 reporter construct in VSMCs is shown. Firefly luciferase values were normalized to Renilla luciferase activity and plotted as relative luciferase activity. C. RT-PCR analysis was performed to examine the effects of miR-541 mimic on IRF7 expression in VSMCs. Ectopic expression of miR-541 significantly decreased IRF7 transcripts. GAPDH was used as internal control. D. Western blotting was performed to examine the effect of miR-541 mimic on the expression of IRF7. GAPDH was also detected as a loading control. E. Inhibition of miR-541 promoted the protein expression of IRF7. F. RT-PCR analysis was performed to examine the effects of miR-541 inhibitor on IRF7 expression in VSMCs. ***p<0.001.