Analysis of the expression of IRAK-1, NF-κB and MAPK proteins by Western blotting. A: Analysis of IRAK-1, NF-κB and MAPK proteins in H. pylori free macrophages with miR-223 mimics, miR-223 inhibitors or negative miRNA treatment. B: Analysis of IRAK-1, NF-κB and MAPK proteins in H. pylori infected macrophages with miR-223 mimics, miR-223 inhibitors or negative miRNA treatment. Data shown are mean ± SEM of 3 independent experiments (*P<0.045).