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. 2016 Feb 15;8(2):719–731.

Figure 2.

Figure 2

MiR-99ainhibits proliferation and invasion of EC cells. A. RT-PCR was performed to detect the expression of miR-99a in GC cell lines (HEC-1B and ishikawa) after treatment with mimics (normalized to U6); B. CCK-8 was performed to analyze the effect of miR-99a on cell proliferation of HEC-1B and ishikawa cells; C, D. The influences of miR-99a on cell cycle distribution and apoptosis of HEC-1B and ishikawa cells were analyzed using fluorescence-activated cell sorting (FACS); E. The effects of miR-99a on cell invasion were detected using transwell chamber assays. The left panel showed representative photos of cells passing through the membrane; the right panel showed the statistic data of cells passing through the membrane; *P<0.05; **P<0.01.