Table 1.

Primer sequences for PCR or Reverse transcription. F for forward, R for reverse

Gene	Primer Sequence
Primers for Reverse transcription
miR-99a	5’-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGCACAAG-3’
U6	5’-AAAATATGGAACGCTTCACGAATTTG-3’
AKT1	5’-TTTTTTTTTTTTTTTTTT-3’(Oligo(dT))
mTOR	5’-TTTTTTTTTTTTTTTTTT-3’(Oligo(dT))
GAPDH	5’-TTTTTTTTTTTTTTTTTT-3’(Oligo(dT))
Primers for quantitative Real-time PCR
miR-99a-F	5’-GCGAACCCGTAGATCCGAT-3’
miR-99a-R	5’-CAGTGCAGGGTCCGAGGT-3’
U6-F	5’-CTCGCTTCGGCAGCACATATACT-3’
U6-R	5’-ACGCTTCACGAATTTGCGTGTC-3’
AKT1-F	5’-CTCTTTCCAGACCCACGAC-3’
AKT1-R	5’-CCAGGGCTGACACAATCTC-3’
GAPDH-F	5’-TCAACGACCACTTTGTCAAGCTCA-3’
GAPDH-R	5’-GCTGGTGGTCCAGGGGTCTTACT-3’
mTOR-F	5’-CCATCAGCGTCCCTACCTT-3’
mTOR-R	5’-GAGAATCAGACAGGCACGAA-3’