Figure 7.

PRDX1 depletion promoted NF-κB p65 activation in SW480 cells. After 24 h of siPRDX1 or siScramble transfection, the protein expressions of NF-κB p65 in cytosol and in nuclear fragments of SW480 cells were analyzed by western blot. A. Protein levels of NF-κB p65 in cytosol extracts and p-NF-κB p65 in nuclear extracts. β-actin and lamin B were used as loading controls. B. Relative density of NF-κB p65 normalized to β-actin. C. Relative quantity of p-NF-κB p65 normalized to lamin B. All figures are representative of three independent experiments performed in triplicate. Data are expressed as means ± SD. Statistical significance: *P < 0.05 vs Mock and siScramble groups; #P < 0.01 vs Mock and siScramble groups.